fatemeh sarlak; Arash Kheradmand; Alireza Rocky; Majid Khaldari
Volume 24, Issue 1 , April 2022, , Pages 109-116
Abstract
The aim of this study was to investigate the changes in some blood parameters in 15 Lori ewes during pregnancy and compare it with one month before pregnancy and lactation. Blood samples were taken from the jagulur vein before normal mating as a control, at the end of every three months of pregnancy ...
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The aim of this study was to investigate the changes in some blood parameters in 15 Lori ewes during pregnancy and compare it with one month before pregnancy and lactation. Blood samples were taken from the jagulur vein before normal mating as a control, at the end of every three months of pregnancy and two weeks after delivery during lactation, and the complete blood cell count of each sample was evaluated by a veterinary cell counter. In extensions, Giemsa staining and differential white blood cell count were performed and total plasma protein values were evaluated by refractometry. The number of red blood cells and the percentage of hematocrits during lactation were significantly reduced compared to pregnancy (P˂0.05). Mean cellular volume was reported in all periods in one spectrum. Decreases in mean hemoglobin levels of red blood cells, mean hemoglobin concentrations in red blood cells and platelet count in pregnancy were observed compared to before mating. The number of leukocytes and neutrophils increased around delivery and decreased after delivery. During pregnancy, the number of eosinophils increased significantly and decreased after delivery (P˂0.05). The number of monocytes and lymphocytes increased in late pregnancy and decreased during lactation (P˂0.05). The amount of total protein in the plasma decreased significantly from 150-100 days of gestation (P˂0.05). Thus, it is concluded that monitoring of changes in blood parameters of Lori ewes, dietary adjustment and preventive measures are necessary to prevent blood disorders such as anemia and thrombocytopenia.
aref kord; Arash Kheradmand; Alireza Rocky; Majid Khaldari
Volume 23, Issue 4 , January 2022, , Pages 515-524
Abstract
The aim of this study was to investigate the changes of some blood biochemical parameters in Lori-Bakhtiari ewes during different months of pregnancy and the influence of fetus numbers on these parameters. For this purpose, 20 ewes of Lori Bakhtiari breed were selected and mated with fertile rams. One ...
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The aim of this study was to investigate the changes of some blood biochemical parameters in Lori-Bakhtiari ewes during different months of pregnancy and the influence of fetus numbers on these parameters. For this purpose, 20 ewes of Lori Bakhtiari breed were selected and mated with fertile rams. One month before mating and in the first to fifth months of pregnancy blood samples were collected from ewes and biochemical and enzymatic parameters including calcium, phosphorus, magnesium, urea, creatinine, total bilirubin, AST, ALP and GGT were measured. The concentration of calcium and AST activity were constant at the times of the study, but they increased in the last month of pregnancy (P<0.05). Magnesium concentration increased in second month of pregnancy (P<0.05) and phosphorus concentration was decreased during pregnancy compared to the month before mating (P<0.05). Creatinine concentration decreased in the second and third months of pregnancy compared to other months of pregnancy (P<0.05). Total bilirubin and urea concentrations increased during fourth month and the second half of pregnancy, respectively (P<0.05). GGT activity was almost stable during study period but decreased in the fourth month of pregnancy (P<0.05), and ALP enzyme activity showed increasing trend during pregnancy period (P<0.05). The mean concentration of the studied parameters in singleton ewes was not significantly different from twins (P <0.05). Based on the results of this study, blood biochemical changes in Lori Bakhtiari ewes can be used to assess their physiological status during pregnancy.
Fatemeh Yarahmadi; Ali Kiani; Raziye Dekami; Hamidreza Sahraie; Ayoub Azizi; Alireza Rocky
Volume 22, Issue 1 , March 2020, , Pages 37-45
Abstract
The present study aimed to investigate the effect of dietary betaine supplementation on circulating homocysteine, antioxidant enzymes and lipid peroxidation in pregnant Sanjabi ewes. Twenty multiparous pregnant ewes (3-5 years old) were fed either a basal diet (Control: 71.2±3.6 kg BW) or the ...
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The present study aimed to investigate the effect of dietary betaine supplementation on circulating homocysteine, antioxidant enzymes and lipid peroxidation in pregnant Sanjabi ewes. Twenty multiparous pregnant ewes (3-5 years old) were fed either a basal diet (Control: 71.2±3.6 kg BW) or the basal diet supplemented with five g per kg dry matter betaine (Betaine: 71.6±3.8 kg BW) during the last five weeks of gestation in a completely random design. Feed intake, body weight (BW) and body condition score (BCS) were weekly recorded. Blood samples were taken from the jugular vein at 28, 14, seven, and one-day pre-partum. The activity of glutathione peroxidase (GPx), catalase (CAT) and blood concentration of homocysteine, and malondialdehyde (MDA) were determined. Dry matter intake, BCS, and BW of ewes were not affected by betaine consumption. In both groups, the activity of GPx increased (P<0.05), while CAT and MDA decreased (P<0.05) as gestation progressed. The activity of GPx and CAT remained unchanged by betaine. Ewes receiving betaine showed greater (P<0.05) blood homocysteine, but lower (P<0.05) MDA as compared to the control ewes. The birth weight of lambs in Betaine ewes was heavier (P=0.06) than those of control ewes. In conclusion, dietary supplementation of betaine was beneficial for alleviating oxidative stress and improving lambs' birth weight in Sanjabi ewes.